Microscope Resolution 2.04

Characterize your microscope objective lenses to evaluate their compatibility with your microscope's image acquisition system. The application accommodates both transmitted light objectives and fluorescence objectives. Input details such as magnification, immersion medium (air, oil, glycerol, or silicone oil), numerical aperture (NA), condenser NA, and light wavelength (lambda) to determine the lateral resolution, axial resolution, and brightness of the objective. Assess your acquisition system parameters (camera pixel size, camera binning, and additional magnification) to ensure sampling at Nyquist frequency. Save and easily recall individual objectives, and conveniently share them with fellow microscopists through email, MMS, LinkedIn, or Facebook. Display the equations utilized for calculations, with referenced sources available in the "Information" section.

• Theoretical Resolution is computed based on Ernst Abbe's renowned equation (Abbe 1873).
• Actual Resolution is determined using 'The Rayleigh Criterion'.
• Axial Resolution for fluorescence objectives follows calculations outlined in Inoue's chapter of Jim Pawley's “Handbook of Biological Confocal Microscopy” (3rd edition 2006).
• Camera pixel sizes can be selected from the predefined camera list; alternatively, manual entry is possible if your camera model is not listed.
• Utilize the 'share' functionality to disseminate all outputs via email, MMS, or LinkedIn.
• Whether your microscope originates from Zeiss, Evos, Leica, Olympus, Nikon, Motic, or Meiji Techno, the underlying principles remain consistent, rendering the app beneficial across various microscope brands.

The Camera dialog provides a comprehensive list of compatible cameras from manufacturers such as Andor, Hamamatsu, Photometrics, PCO, QImaging, Leica, Lumenera, Olympus, Nikon, Motic, and Zeiss.